How much caffeine per day? The EFSA limit, drinks compared, and what your genes have to do with it
ca. 15 Minuten

The key points
Up to 400 mg of caffeine per day, spread throughout the day, poses no safety concerns for healthy adults. That is the assessment of the European Food Safety Authority, paraphrased from EFSA 2015. For a single dose, the same assessment applies up to 200 mg. This refers to total intake from all sources.
400 mg is about four 200 ml cups of filter coffee. Or five espressos. Or five 250 ml energy drinks. This article converts the number into servings, compares the caffeine content of individual drinks, and explains where different amounts apply: for sleep, during pregnancy, and for children.
Chapter 1 gives the numbers, Chapter 2 converts them into cups, and Chapter 3 brings the drinks together in a table. If you want to know what genetics has to do with the subject, skip to Chapter 7. Chapter 8 is a guide to tracking your intake for a single day.
What to expect in this article
1. How much caffeine per day is considered safe?
2. What 400 mg of caffeine means in everyday life
3. Caffeine per serving: coffee, tea, energy drinks, cola, chocolate
4. Red Bull and cola: the amounts, without judgment
5. Sleep, pregnancy, children: where different amounts apply
6. Three myths about caffeine
7. What a DNA test can tell you about caffeine
8. Keep track for one day
Frequently asked questions
Sources
How much caffeine per day is considered safe?
Caffeine is the substance found in coffee, tea, dark chocolate, cola, and energy drinks. A government agency has answered the question of how much of it is okay per day: EFSA, the European Food Safety Authority, which advises the EU on food matters based on scientific evidence. It published an assessment of caffeine in 2015, citing several amounts.
The wording matters. EFSA does not recommend that anyone drink caffeine. It only says up to what amount it sees no cause for safety concerns. This is not a seal of approval or a daily target, but an upper limit without concerns.
400 mg
Total daily intake, spread throughout the day, without safety concerns for healthy adults (paraphrased from EFSA 2015).
200 mg
as a single dose, meaning consumed all at once rather than spread throughout the day, also without safety concerns (paraphrased from EFSA 2015).
200 mg
per day from all sources during pregnancy and breastfeeding (paraphrased from EFSA 2015).
100 mg
as a single dose close to bedtime, it may affect sleep duration and sleep patterns in some adults (paraphrased from EFSA 2015).
Total intake means everything added together
400 mg is not a coffee limit. It means everything you consume over the course of a day: coffee, black tea, the cola in the afternoon, the energy drink before your workout, the dark chocolate in the evening. If you only count coffee, you’re undercounting.
For children and adolescents, the EFSA gives a different way of calculating intake: 3 mg per kg of body weight per day, meaning milligrams per kilogram of body weight, an amount that increases with weight rather than being the same for everyone (paraphrased from EFSA 2015). For adults, 400 mg is equivalent to about 5.7 mg per kg of body weight, while 200 mg as a single dose is about 3 mg per kg (EFSA 2015).
The most common objection at this point is: I tolerate coffee well, so this doesn't affect me. That may be true. Even so, something else is decisive: the figure doesn't describe how you feel, but an amount below which the authority has expressed no safety concerns. If you lie awake at night, the same assessment includes the guidance on 100 mg before bed.
What 400 mg of caffeine means in everyday life
Cups don't list milligrams. Still, you can convert the figure because the EFSA provides example amounts for individual servings in the same publication. This turns an abstract limit into a number of cups.
The calculation for filter coffee: one 200 ml cup contains 90 mg in the example (EFSA 2015). 400 mg divided by 90 mg per cup equals 4.4 - so about four cups a day. For espresso: 400 mg divided by 80 mg per 60 ml cup equals exactly five. For energy drinks: 400 mg divided by 80 mg per 250 ml can also equals five.
These figures are calculation aids, not measurements from your kitchen. The caffeine content of coffee varies depending on the preparation, beans, and amount of grounds. The EFSA examples give you a rough idea that you can use to estimate your daily intake without weighing anything.
At a glance
400 mg is equivalent to about four 200 ml cups of filter coffee - calculated as 400 divided by 90 mg per cup, according to EFSA 2015, total intake from all sources.
Or five 60 ml espressos - calculated as 400 divided by 80 mg per cup, according to EFSA 2015, total intake from all sources.
Or five 250 ml energy drinks - calculated as 400 divided by 80 mg per can, according to EFSA 2015, total intake from all sources.
Caffeine per serving: coffee, tea, energy drinks, cola, chocolate
The following table compares EFSA example servings with two manufacturer specifications. The last column shows what share of the 400 mg daily amount one serving accounts for. For a closer look at black tea, see the article on caffeine content in black tea.
| Serving | Caffeine in mg | Source and year | Share of 400 mg |
|---|---|---|---|
| Espresso, 60 ml | 80 mg | according to EFSA 2015 | about 20 percent |
| Filter coffee, 200 ml | 90 mg | according to EFSA 2015 | about 23 percent |
| Black tea, 220 ml | 50 mg | according to EFSA 2015 | about 13 percent |
| Energy drink, 250 ml | 80 mg | according to EFSA 2015 | about 20 percent |
| Red Bull, 250 ml can | 80 mg | Red Bull GmbH, manufacturer information 2026 | about 20 percent |
| Cola, 355 ml | 40 mg | according to EFSA 2015 | around 10 percent |
| Coca-Cola, per 100 ml | 10 mg | Coca-Cola, manufacturer information, 2026 | around 2.5 percent |
| Dark chocolate, 50 g | 25 mg | according to EFSA 2015 | around 6 percent |
Counting rule for the last column: caffeine per serving divided by 400 mg, rounded to the nearest half percentage point. The amounts are example servings and manufacturer information, not measurements of your drink.
Red Bull and cola: amounts, without judgment
There is manufacturer information for two drinks. It is shown here as an amount, not an assessment.
Red Bull lists 80 mg of caffeine for the 250-ml can and says that this is about the amount in a cup of coffee (manufacturer information, 2026). For larger cans, the manufacturer lists 105 mg for 330 ml, 114 mg for 355 ml and 151 mg for 473 ml. Two large 473-ml cans therefore contain 302 mg, calculated as 151 mg times two.
In its answers to frequently asked customer questions, Coca-Cola lists 10 mg of caffeine per 100 ml (manufacturer information, 2026). For a 330-ml can, that means: 10 mg per 100 ml times 3.3 equals around 33 mg. The manufacturer puts the amount in context itself.
Cited source
The manufacturer writes that “a can of Coca-Cola with 10 mg of caffeine per 100 ml contains significantly less caffeine than the same amount of coffee.”
Coca-Cola, manufacturer information
Answers to frequently asked customer questions, 2026
What does this mean for your day? Just the arithmetic. A 330-ml can of cola with around 33 mg accounts for less than a tenth of the 400 mg, while a 250-ml can of Red Bull with 80 mg accounts for around a fifth. None of these figures says anything about taste, sugar or benefits.
Sleep, pregnancy, children: when different figures apply
The 400 mg figure applies to healthy adults. EFSA gives separate figures for three groups and one time of day, and they are lower.
Regarding sleep: single doses of 100 mg of caffeine can affect sleep duration and patterns in some adults, especially close to bedtime (paraphrased from EFSA 2015). 100 mg is a little more than a 200 ml cup of filter coffee. If you have trouble falling asleep in the evening but think the espresso after dinner is harmless, here is a figure you can check. The relationship between genetics and sleep is discussed in the article on genes, sleep and caffeine.
During pregnancy and breastfeeding, EFSA cites 200 mg per day from all sources as an amount of no safety concern (paraphrased from EFSA 2015). The assessment says no more than that, so neither does this article.
For children and adolescents, EFSA suggests 3 mg per kg of body weight per day as a safety level for habitual consumption (paraphrased from EFSA 2015). The figure increases with weight, which is exactly why there is no calculation example here: the relevant reference is the individual child’s weight, not an average.
Key takeaway
It’s not just the total amount for the day that matters, but also the timing: 400 mg spread throughout the day and 100 mg shortly before bedtime are two different points in the same assessment (paraphrased from EFSA 2015).
A practical side note: Before a blood test, separate rules apply to what you’re allowed to drink. You can find out what’s permitted in the article Fasting Before a Blood Test.
Three myths about caffeine
EFSA’s example caffeine contents dispel a few persistent assumptions. Each of the three corrections below is based on the same figures as the table.
Myth
Espresso has more caffeine than filter coffee.
What the numbers say
Per serving, it’s the other way around: a 60 ml espresso contains 80 mg in this example, while a 200 ml cup of filter coffee contains 90 mg (according to EFSA 2015). Espresso is more concentrated, but the serving is smaller.
Myth
Energy drinks are much stronger than coffee.
What the numbers say
A 250 ml can contains 80 mg (according to EFSA 2015), and the manufacturer of Red Bull also lists 80 mg for the same can size (manufacturer’s information, 2026). That’s less than the 90 mg in a 200 ml cup of filter coffee.
Myth
If you tolerate coffee well, caffeine won’t cause you sleep problems.
What the numbers say
Even single doses of 100 mg close to bedtime can affect sleep duration and sleep patterns in some adults (paraphrased from EFSA 2015). Tolerating it well during the day and watching the clock are two different things.
What a DNA test reveals about caffeine
So far, we’ve covered amounts that are the same for everyone. Readers who come across this topic often have a different question: Does my body have anything to do with it? Here, mybody®x (MYBODY Lab GmbH) offers a starting point, not an answer about tolerance.
The INFINITY DNA test includes a report titled “Caffeine Metabolism” in the “Metabolic Traits” chapter. It looks at the CYP1A2 gene, a section of genetic material that contains the instructions for making a protein. The same chapter also includes reports on alcohol metabolism, lactose metabolism, and gluten intolerance.
And here’s the limit, frankly: the report doesn’t predict how much coffee you can tolerate, and it doesn’t replace EFSA figures. It looks at this one gene and is intended as a starting point for dietary and lifestyle advice. It says nothing more about your tolerance.
The EFSA limit applies to everyone. Your gene report is a starting point, not a second limit.
Who it’s for: readers who already want to know what their genetic makeup can tell them about nutrition and who see the caffeine report as one part of that. If you’re only looking for a daily amount, the table above is enough.
DNA analysis from saliva
INFINITY DNA Test | Includes a 28-day plan & recipe book
DNA analysis, meaning laboratory testing of your genetic makeup, from a saliva sample you collect yourself at home. No blood, no clinic visit. In the “Metabolic Traits” chapter, the test includes the “Caffeine Metabolism” report on the CYP1A2 gene, as well as reports on alcohol metabolism, lactose metabolism, and gluten intolerance. What it doesn’t do: predict how well you’ll tolerate caffeine, make a diagnosis, or predict disease.
Track your intake for one day
The most useful exercise on this topic takes exactly one day. Write down what you drink and the time you drink it. Then use the table in chapter 3 and note the milligrams for each serving.
Then compare two numbers. Your daily total against 400 mg, and your last serving before bedtime against the 100 mg in the sleep guidance (in line with EFSA 2015). The time of day is the part that’s easy to overlook - you can figure it out in a single day without giving anything up.
Back to the beginning of this article: The question was how much caffeine per day is okay. The honest answer has two parts. A number for everyone, which you can convert into four cups. And a time of day that only your own routine can answer. If you’d like to know how coffee fits into the bigger picture, continue reading about whether coffee is healthy.
Frequently asked questions
How much caffeine is in a can of Red Bull?
A 250 ml can contains 80 mg of caffeine, which the manufacturer’s 2026 information says is about the same as a cup of coffee. For larger cans, the manufacturer lists 105 mg for 330 ml, 114 mg for 355 ml, and 151 mg for 473 ml. Compared with the 400 mg that EFSA’s 2015 assessment says healthy adults can consume per day without safety concerns, the small can accounts for around 20 percent and the 473 ml can around 38 percent.
How much caffeine is in cola?
Coca-Cola lists 10 mg of caffeine per 100 ml (manufacturer’s information, 2026). For a 330 ml can, that comes to around 33 mg, calculated as 10 mg per 100 ml multiplied by 3.3. In its 2015 assessment, EFSA gives 40 mg per 355 ml of cola as an example serving. Both figures are well below the 90 mg per 200 ml example serving of filter coffee cited by EFSA.
How many cups of coffee is 400 mg of caffeine?
About four 200 ml cups of filter coffee, calculated as 400 mg divided by 90 mg per cup (example caffeine content according to EFSA 2015). For 60 ml espresso shots, that’s five shots, calculated as 400 divided by 80 mg. The figure applies to total intake from all sources, including tea, cola, energy drinks, and dark chocolate. The actual strength of coffee depends on how it is prepared.
Does the 400 mg limit also apply during pregnancy?
No. For pregnant and breastfeeding women, EFSA gives a lower figure: 200 mg per day from all sources, spread throughout the day, is not considered a safety concern (paraphrased from EFSA 2015). That is half the amount for healthy adults. The assessment gives no further guidance; questions about your individual situation should be discussed with your doctor or midwife.
What does a DNA test tell you about caffeine, and what doesn’t it tell you?
The mybody®x INFINITY DNA test includes the report “Caffeine metabolism” in the “Metabolic traits” chapter and looks at the CYP1A2 gene. The report is intended for nutrition and lifestyle guidance. It does not predict how much coffee you can tolerate, provide a diagnosis, or replace EFSA’s intake guidelines. The genetic predisposition describes a probability across population groups, not a fixed outcome for you.
Next step
Count first, then test
You can work out your daily intake with a pen and paper. If you’d also like to know what your genes can tell you about your metabolism, the INFINITY DNA test includes a report for that.
View the INFINITY DNA test Genes, sleep, and caffeineRead more
You might also be interested in
A direct comparison of the two drinks, beyond their caffeine content.
What coffee has to do with digestion.
What genes have to do with sleep rhythms and where the limits lie.
Sources
- European Food Safety Authority (EFSA): Caffeine topic page for the Scientific Opinion on the safety of caffeine, EFSA Journal 2015;13(5):4102, DOI 10.2903/j.efsa.2015.4102 (as of 2015) – efsa.europa.eu
- Red Bull GmbH: Manufacturer's information on caffeine content, answers to frequently asked customer questions (as of 2026) – redbull.com
- Coca-Cola (Austria): Manufacturer's information on caffeine content, answers to frequently asked customer questions (as of 2026) – coca-cola.com
All quantities for daily amounts, individual doses, pregnancy, children, and sleep, as well as the example amounts per serving, are based on [1], rendered in German in substance. The information about Red Bull comes from [2], and the information about Coca-Cola from [3]. Information about the INFINITY DNA test comes from mybody®x product pages and was checked on 14.09.2026.
mybody®x Editorial & Expert Team
Nutrition Metabolism Genetics
This article was prepared by the mybody®x expert team and reviewed against the sources cited. Find out who’s behind it on the Authors page.
Published on 14.09.2026 · Last updated on 14.09.2026
The content is for general information and does not replace medical advice, diagnosis, or treatment. Reference ranges depend on the laboratory, method, and age - the information on your report is always authoritative.
DNA analysis is intended to provide nutrition and lifestyle advice. It is not a diagnostic procedure, does not predict disease, and does not replace a medical examination or consultation. Genetic variants describe probabilities in population groups, not determinations for individuals.
Our products are not intended to diagnose, treat, cure, or prevent diseases.
